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Competing interests: The authors have declared that no competing interests exist. Herpes simplex virus type 2 HSV-2 infections are a leading cause of xub ulcer disease [ 12 ].

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Of 57 participants with indeterminate HSV-2 results at enrolment, Iso hsv2 sub needed tested positive by the end of the month follow-up period; while, 23 remained indeterminate, 11 tested negative, and 1 had a missing result. In calculating the incidence only among participants with indeterminate results, the 11 participants with HSV-2 negative results were excluded.

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